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twist2  (Proteintech)


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    Proteintech twist2
    Knockdown of CLEC18B suppresses glioma cell migration and invasion via Wnt/β-Catenin/EMT signaling. (A) Transwell migration and invasion assays showing reduced migratory and invasive abilities of U87 and U251 cells after CLEC18B knockdown. (B) Correlation between CLEC18B expression and Wnt/β-Catenin/EMT pathway gene expression in TCGA datasets. (C) Western blot analysis showing that CLEC18B knockdown suppresses the expression of mesenchymal markers (N-cadherin, Slug, Twist1, <t>Twist2,</t> Snail, and β-catenin) and promotes the expression of the epithelial marker E-cadherin.
    Twist2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+twist2/TWIST2+Antibody/pmc12410489-124-28-37
    Average 93 stars, based on 23 article reviews
    twist2 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "SP1-Mediated upregulation of CLEC18B promotes the proliferation and metastasis of glioma through regulation of the Wnt/β-Catenin/EMT Pathway"

    Article Title: SP1-Mediated upregulation of CLEC18B promotes the proliferation and metastasis of glioma through regulation of the Wnt/β-Catenin/EMT Pathway

    Journal: Translational Oncology

    doi: 10.1016/j.tranon.2025.102515

    Knockdown of CLEC18B suppresses glioma cell migration and invasion via Wnt/β-Catenin/EMT signaling. (A) Transwell migration and invasion assays showing reduced migratory and invasive abilities of U87 and U251 cells after CLEC18B knockdown. (B) Correlation between CLEC18B expression and Wnt/β-Catenin/EMT pathway gene expression in TCGA datasets. (C) Western blot analysis showing that CLEC18B knockdown suppresses the expression of mesenchymal markers (N-cadherin, Slug, Twist1, Twist2, Snail, and β-catenin) and promotes the expression of the epithelial marker E-cadherin.
    Figure Legend Snippet: Knockdown of CLEC18B suppresses glioma cell migration and invasion via Wnt/β-Catenin/EMT signaling. (A) Transwell migration and invasion assays showing reduced migratory and invasive abilities of U87 and U251 cells after CLEC18B knockdown. (B) Correlation between CLEC18B expression and Wnt/β-Catenin/EMT pathway gene expression in TCGA datasets. (C) Western blot analysis showing that CLEC18B knockdown suppresses the expression of mesenchymal markers (N-cadherin, Slug, Twist1, Twist2, Snail, and β-catenin) and promotes the expression of the epithelial marker E-cadherin.

    Techniques Used: Knockdown, Migration, Expressing, Gene Expression, Western Blot, Marker

    Related Articles

    Blocking Assay:

    Article Title: Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.
    Article Snippet: .. After blocking with 5% normal goat serum, the slices were incubated with anti-ACOT12 (Thermo, MA5-25428), anti-TWIST2 (Proteintech, 11752-1-AP), anti-vimentin (Proteintech, 10366- 1-AP), anti-N-cadherin (Proteintech, 22018-1-AP) or anti-Ki67 (Proteintech, 27309-1-AP) overnight at 4 °C and washed with PBS 3 times. ..

    Article Title: Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12
    Article Snippet: .. After blocking with 5% normal goat serum, the slices were incubated with anti-ACOT12 (Thermo, MA5-25428), anti-TWIST2 (Proteintech, 11752-1-AP), anti-vimentin (Proteintech, 10366-1-AP), anti-N-cadherin (Proteintech, 22018-1-AP) or anti-Ki67 (Proteintech, 27309-1-AP) overnight at 4 °C and washed with PBS 3 times. ..

    Incubation:

    Article Title: Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.
    Article Snippet: .. After blocking with 5% normal goat serum, the slices were incubated with anti-ACOT12 (Thermo, MA5-25428), anti-TWIST2 (Proteintech, 11752-1-AP), anti-vimentin (Proteintech, 10366- 1-AP), anti-N-cadherin (Proteintech, 22018-1-AP) or anti-Ki67 (Proteintech, 27309-1-AP) overnight at 4 °C and washed with PBS 3 times. ..

    Article Title: Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12
    Article Snippet: .. After blocking with 5% normal goat serum, the slices were incubated with anti-ACOT12 (Thermo, MA5-25428), anti-TWIST2 (Proteintech, 11752-1-AP), anti-vimentin (Proteintech, 10366-1-AP), anti-N-cadherin (Proteintech, 22018-1-AP) or anti-Ki67 (Proteintech, 27309-1-AP) overnight at 4 °C and washed with PBS 3 times. ..

    Article Title: A Comparative Analysis Dissecting the Immune Landscape of Vitiligo and Melanoma from a single-cell Perspective: Two Sides of the Same Coin?
    Article Snippet: .. Sections were permeabilized with 0.2% Triton X-100 (Sigma-Aldrich) and then blocked with 10% normal goat serum (Sigma-Aldrich) and incubated overnight at 4 °C with the following primary antibody: anti-PDGFRA (1: 200, MAB322, Biotechne), anti-TWIST2 (1: 500, 66544-Ig, Proteintech), anti-IL-6 (1: 200, 66146-Ig, Proteintech), anti-CXCL9 (1: 100, ab290643, Abcam), anti-KRT5 (1: 100, ab64081, Abcam), anti-S100A8 (1: 500, ab92331, Abcam), anti-CXCL10 (1: 1000, ab306587, Abcam), anti-CD8 (1: 100, ab237709, Abcam), anti-CD69 (1: 200, 10803-1-AP, Proteintech), anti-CD45RO (1: 200, 65150-1-Ig, Proteintech), anti-IL-2R (1: 400, MAB623-SP, Biotechne), anti-CXCR3 (1: 500, ab288437, Abcam), anti-MelanA (1: 1000, ab187369, Abcam), anti-CD11B (1: 400, ab133357, Abcam), anti-CCR7 (1: 200, MAB197-SP, Biotechne), anti-CCL7 (1: 100, K108742P, Solarbio). ..

    Article Title: A Comparative Analysis Dissecting the Immune Landscape of Vitiligo and Melanoma from a single-cell Perspective: Two Sides of the Same Coin?
    Article Snippet: .. Sections were permeabilized with 0.2% Triton X-100 (Sigma-Aldrich) and then blocked with 10% normal goat serum (Sigma-Aldrich) and incubated overnight at 4 °C with the following primary antibody: anti-PDGFRA (1: 200, MAB322, Biotechne), anti-TWIST2 (1: 500, 66544-Ig, Proteintech), anti-IL-6 (1: 200, 66146-Ig, Proteintech), anti-CXCL9 (1: 100, ab290643, Abcam), anti-KRT5 (1: 100, ab64081, Abcam), anti-S100A8 (1: 500, ab92331, Abcam), anti-CXCL10 (1: 1000, ab306587, Abcam), anti-CD8 (1: 100, ab237709, Abcam), anti-CD69 (1: 200, 10803-1-AP, Proteintech), anti-CD45RO (1: 200, 65150-1-Ig, Proteintech), anti-IL2R (1: 400, MAB623-SP, Biotechne), anti-CXCR3 (1: 500, ab288437, Abcam), anti-MelanA (1: 1000, ab187369, Abcam), anti-CD11B (1: 400, ab133357, Abcam), antiCCR7 (1: 200, MAB197-SP, Biotechne), anti-CCL7 (1: 100, K108742P, Solarbio). ..

    Quantitative RT-PCR:

    Article Title: Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.
    Article Snippet: .. Oligos and antibodies used in the research. miR-155-5p-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCCCT For miR-155-5p RT-qPCR miR-155-5p-F GCGCTTAATGCTAATCGTGAT miR-155-5p-R CCAGTGCAGGGTCCGAGGTA miR-574-5p-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACACAC For miR-574-5p RT-qPCR miR-574-5p-F GGCTGAGTGTGTGTGTGTGA miR-574-5p-R CCAGTGCAGGGTCCGAGGTA miR-187-3p-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCGGCT For miR-187-3p RT-qPCR miR-187-3p-F GGCTCGTGTCTTGTGTTGC miR-187-3p-R CCAGTGCAGGGTCCGAGGTA miR-130a-3p-T GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATGCCC For miR-130a-3p RT-qPCR miR-130a-3p-F GCGCCAGTGCAATGTTAAAA miR-130a-3p-R CCAGTGCAGGGTCCGAGGTA miR-181b-5p-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCCAC For miR-181b-5p RT-qPCR miR-181b-5p-F GCAACATTCATTGCTGTCG miR-181b-5p-R CCAGTGCAGGGTCCGAGGTA miR-361-5p-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTACCC For miR-361-5p RT-qPCR miR-361-5p-F GCGCTTATCAGAATCTCCAG miR-361-5p-R CCAGTGCAGGGTCCGAGGTA miR-16-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGCCAA For miR-16 RT-qPCR miR-16-F GCGCGCTAGCAGCACGTAAATA miR-16-R CCAGTGCAGGGTCCGAGGTA GAPDH-F GCACCGTCAAGGCTGAGAAC For GAPDH RT-qPCR GAPDH-R TGGTGAAGACGCCAGTGGA U6-F CTCGCTTCGGCAGCACA For U6 RT-qPCR U6-R AACGCTTCACGAATTTGCGT ACOT12-F TGCTGGAGTTTCCTGCGTTAC For ACOT12 RT-qPCR ACOT12-R GCATATCCTGTACCATGACCTTG TWIST2-F ACAGCAGTGACATCGGACAG For TWIST2 RT-qPCR TWIST2-R CCCCAAACATAAGACCCAGA Vimentin-F GAGAACTTTGCCGTTGAAGC For Vimentin RT-qPCR Vimentin-R GCTTCCTGTAGGTGGCAATC N-cadherin-F ACAGTGGCCACCTACAAAGG For N-cadherin RT-qPCR N-cadherin-R CCGAGATGGGGTTGATAATG Cell Death and Disease (2022) 13:725 antibodies were used: anti-CD63 (Abcam, Ab213090); anti-CD9 (Abcam, Ab92726); anti-GM130 (Abcam, Ab52649); anti-ACOT12 (Thermo, MA525428); anti-TWIST2 (Proteintech, 11752-1-AP); anti-vimentin (Proteintech, 10366-1-AP); anti-N-cadherin (Proteintech, 22018-1-AP); and anti-β-actin (Sangon, D110001). ..



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    Knockdown of CLEC18B suppresses glioma cell migration and invasion via Wnt/β-Catenin/EMT signaling. (A) Transwell migration and invasion assays showing reduced migratory and invasive abilities of U87 and U251 cells after CLEC18B knockdown. (B) Correlation between CLEC18B expression and Wnt/β-Catenin/EMT pathway gene expression in TCGA datasets. (C) Western blot analysis showing that CLEC18B knockdown suppresses the expression of mesenchymal markers (N-cadherin, Slug, Twist1, <t>Twist2,</t> Snail, and β-catenin) and promotes the expression of the epithelial marker E-cadherin.
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    Single cell RNA-seq revealed cell lineages and differentiation trajectories of human chondrocytes. A Work flow of single cell RNA-seq. B UMAP of 5 distinct cell types (SC, CDC, PHC, PROC, CPC). C Cell proportions of 5 cell types in all samples. CDC and SC showed decreasing trend while CPC and PHC showed increasing trend. D Dot plot of marker genes in 5 cell types. E Heatmap and representative GO terms of 5 cell types. F Cell lineages predicted by “Slingshot”. G Prediction of cellular dynamics by “sctour”. H Differentiation branches predicted by “monocle2” and cell proportions of 3 main states. One trajectory from CPC to PHC exhibited hypertrophic cellular features and another trajectory from CPC, SC to CDC showed cartilage specific cellular features. I Top ranked TF activities of each cell type and representative TF feature plots of each cell type. J GO terms of TFs enriched in 5 cell types. K Immunofluorescence of LHX6 (CPC), SOX6/SNED1 (SC), SOX9/MGP (CDC), <t>TWIST2/Collagen</t> I (PHC) expression in cartilage niche (n = 3 for each group). SC and CDC account for the majority of chondrocyte in vivo. I (Data are presented as means ± SD; Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test as appropriate; SC: Stromal Cell, CDC: Chondrocyte Differentiated Cell, PHC: Pre-Hypertrophy Cell, PROC: Proliferative Cell, CPC: Chondrocyte Progenitor Cell; ox: oxidative, fc: functional; GO: Gene Ontology; TF, transcription factor; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: no significance)
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    Single cell RNA-seq revealed cell lineages and differentiation trajectories of human chondrocytes. A Work flow of single cell RNA-seq. B UMAP of 5 distinct cell types (SC, CDC, PHC, PROC, CPC). C Cell proportions of 5 cell types in all samples. CDC and SC showed decreasing trend while CPC and PHC showed increasing trend. D Dot plot of marker genes in 5 cell types. E Heatmap and representative GO terms of 5 cell types. F Cell lineages predicted by “Slingshot”. G Prediction of cellular dynamics by “sctour”. H Differentiation branches predicted by “monocle2” and cell proportions of 3 main states. One trajectory from CPC to PHC exhibited hypertrophic cellular features and another trajectory from CPC, SC to CDC showed cartilage specific cellular features. I Top ranked TF activities of each cell type and representative TF feature plots of each cell type. J GO terms of TFs enriched in 5 cell types. K Immunofluorescence of LHX6 (CPC), SOX6/SNED1 (SC), SOX9/MGP (CDC), <t>TWIST2/Collagen</t> I (PHC) expression in cartilage niche (n = 3 for each group). SC and CDC account for the majority of chondrocyte in vivo. I (Data are presented as means ± SD; Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test as appropriate; SC: Stromal Cell, CDC: Chondrocyte Differentiated Cell, PHC: Pre-Hypertrophy Cell, PROC: Proliferative Cell, CPC: Chondrocyte Progenitor Cell; ox: oxidative, fc: functional; GO: Gene Ontology; TF, transcription factor; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: no significance)
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    Image Search Results


    Knockdown of CLEC18B suppresses glioma cell migration and invasion via Wnt/β-Catenin/EMT signaling. (A) Transwell migration and invasion assays showing reduced migratory and invasive abilities of U87 and U251 cells after CLEC18B knockdown. (B) Correlation between CLEC18B expression and Wnt/β-Catenin/EMT pathway gene expression in TCGA datasets. (C) Western blot analysis showing that CLEC18B knockdown suppresses the expression of mesenchymal markers (N-cadherin, Slug, Twist1, Twist2, Snail, and β-catenin) and promotes the expression of the epithelial marker E-cadherin.

    Journal: Translational Oncology

    Article Title: SP1-Mediated upregulation of CLEC18B promotes the proliferation and metastasis of glioma through regulation of the Wnt/β-Catenin/EMT Pathway

    doi: 10.1016/j.tranon.2025.102515

    Figure Lengend Snippet: Knockdown of CLEC18B suppresses glioma cell migration and invasion via Wnt/β-Catenin/EMT signaling. (A) Transwell migration and invasion assays showing reduced migratory and invasive abilities of U87 and U251 cells after CLEC18B knockdown. (B) Correlation between CLEC18B expression and Wnt/β-Catenin/EMT pathway gene expression in TCGA datasets. (C) Western blot analysis showing that CLEC18B knockdown suppresses the expression of mesenchymal markers (N-cadherin, Slug, Twist1, Twist2, Snail, and β-catenin) and promotes the expression of the epithelial marker E-cadherin.

    Article Snippet: They were then incubated overnight at 4 °C with primary antibodies targeting key proteins involved in cell signaling and tumor progression, including SP1, CLEC18B, CDK4, CDK6, β-catenin, Twist1, Twist2, Snail, Slug, N-cadherin, E-cadherin, and β-actin (all from Proteintech).

    Techniques: Knockdown, Migration, Expressing, Gene Expression, Western Blot, Marker

    Single cell RNA-seq revealed cell lineages and differentiation trajectories of human chondrocytes. A Work flow of single cell RNA-seq. B UMAP of 5 distinct cell types (SC, CDC, PHC, PROC, CPC). C Cell proportions of 5 cell types in all samples. CDC and SC showed decreasing trend while CPC and PHC showed increasing trend. D Dot plot of marker genes in 5 cell types. E Heatmap and representative GO terms of 5 cell types. F Cell lineages predicted by “Slingshot”. G Prediction of cellular dynamics by “sctour”. H Differentiation branches predicted by “monocle2” and cell proportions of 3 main states. One trajectory from CPC to PHC exhibited hypertrophic cellular features and another trajectory from CPC, SC to CDC showed cartilage specific cellular features. I Top ranked TF activities of each cell type and representative TF feature plots of each cell type. J GO terms of TFs enriched in 5 cell types. K Immunofluorescence of LHX6 (CPC), SOX6/SNED1 (SC), SOX9/MGP (CDC), TWIST2/Collagen I (PHC) expression in cartilage niche (n = 3 for each group). SC and CDC account for the majority of chondrocyte in vivo. I (Data are presented as means ± SD; Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test as appropriate; SC: Stromal Cell, CDC: Chondrocyte Differentiated Cell, PHC: Pre-Hypertrophy Cell, PROC: Proliferative Cell, CPC: Chondrocyte Progenitor Cell; ox: oxidative, fc: functional; GO: Gene Ontology; TF, transcription factor; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: no significance)

    Journal: Stem Cell Research & Therapy

    Article Title: Blocking calcium-MYC regulatory axis inhibits early dedifferentiation of chondrocytes and contributes to cartilage regeneration

    doi: 10.1186/s13287-025-04483-3

    Figure Lengend Snippet: Single cell RNA-seq revealed cell lineages and differentiation trajectories of human chondrocytes. A Work flow of single cell RNA-seq. B UMAP of 5 distinct cell types (SC, CDC, PHC, PROC, CPC). C Cell proportions of 5 cell types in all samples. CDC and SC showed decreasing trend while CPC and PHC showed increasing trend. D Dot plot of marker genes in 5 cell types. E Heatmap and representative GO terms of 5 cell types. F Cell lineages predicted by “Slingshot”. G Prediction of cellular dynamics by “sctour”. H Differentiation branches predicted by “monocle2” and cell proportions of 3 main states. One trajectory from CPC to PHC exhibited hypertrophic cellular features and another trajectory from CPC, SC to CDC showed cartilage specific cellular features. I Top ranked TF activities of each cell type and representative TF feature plots of each cell type. J GO terms of TFs enriched in 5 cell types. K Immunofluorescence of LHX6 (CPC), SOX6/SNED1 (SC), SOX9/MGP (CDC), TWIST2/Collagen I (PHC) expression in cartilage niche (n = 3 for each group). SC and CDC account for the majority of chondrocyte in vivo. I (Data are presented as means ± SD; Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test as appropriate; SC: Stromal Cell, CDC: Chondrocyte Differentiated Cell, PHC: Pre-Hypertrophy Cell, PROC: Proliferative Cell, CPC: Chondrocyte Progenitor Cell; ox: oxidative, fc: functional; GO: Gene Ontology; TF, transcription factor; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: no significance)

    Article Snippet: All antibodies: MGP, TWIST2, SOX6, Collagen I, GAPDH (Proteintech, California, USA); SNED1 (CUSABIO, Wuhan, China); LHX6 (Santa Cruz Biotechnology, Dallas, TX, USA); SOX9 (Abcam, Massachusetts, USA).

    Techniques: RNA Sequencing, Marker, Immunofluorescence, Expressing, In Vivo, Functional Assay

    Cell passaging experienced various cellular phenotypic changes. A Biomarkers expression in scRNA data among 3 passages. B Protein expression levels by WB. C–F : LHX6 ( C ), TWIST2/Collagen I ( D ), SOX6/SNED1 ( E ) and SOX9/MGP ( F ) immunofluorescence staining for 3 passages. Biomarkers exhibited similar trends to their proportions. G Heatmap, GO and KEGG enrichment analysis of DEGs among 3 passages. H Differentiative score measured by “CytoTrace”. More score represents less differentiative degree. I Proportions of cell cycle phases. J EdU positive cell proportion of 3 cell passages (n = 3 each group). K Oxidative phosphorylation score. L OCR curves of chondrocytes in 3 passages (n = 3 each group). M 6 major parameters of OCR assay for 3 passages. Totally, markers for SC and CDC gradually decreased while those for CPC and PHC increased. P1 cells exhibited more cartilage cellular features. P3 cells for early dedifferentiation exhibited highest aerobic respiration level, proliferative ability and least differentiative degree which were closer to dedifferentiation. P6 cells showed hypertrophic differentiation features. (Data are presented as means ± SD; Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test as appropriate; DEGs: Differentially Expressed Genes; WB: Western Blot; OCR: Oxygen Consumption Rate; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: no significance)

    Journal: Stem Cell Research & Therapy

    Article Title: Blocking calcium-MYC regulatory axis inhibits early dedifferentiation of chondrocytes and contributes to cartilage regeneration

    doi: 10.1186/s13287-025-04483-3

    Figure Lengend Snippet: Cell passaging experienced various cellular phenotypic changes. A Biomarkers expression in scRNA data among 3 passages. B Protein expression levels by WB. C–F : LHX6 ( C ), TWIST2/Collagen I ( D ), SOX6/SNED1 ( E ) and SOX9/MGP ( F ) immunofluorescence staining for 3 passages. Biomarkers exhibited similar trends to their proportions. G Heatmap, GO and KEGG enrichment analysis of DEGs among 3 passages. H Differentiative score measured by “CytoTrace”. More score represents less differentiative degree. I Proportions of cell cycle phases. J EdU positive cell proportion of 3 cell passages (n = 3 each group). K Oxidative phosphorylation score. L OCR curves of chondrocytes in 3 passages (n = 3 each group). M 6 major parameters of OCR assay for 3 passages. Totally, markers for SC and CDC gradually decreased while those for CPC and PHC increased. P1 cells exhibited more cartilage cellular features. P3 cells for early dedifferentiation exhibited highest aerobic respiration level, proliferative ability and least differentiative degree which were closer to dedifferentiation. P6 cells showed hypertrophic differentiation features. (Data are presented as means ± SD; Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test as appropriate; DEGs: Differentially Expressed Genes; WB: Western Blot; OCR: Oxygen Consumption Rate; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: no significance)

    Article Snippet: All antibodies: MGP, TWIST2, SOX6, Collagen I, GAPDH (Proteintech, California, USA); SNED1 (CUSABIO, Wuhan, China); LHX6 (Santa Cruz Biotechnology, Dallas, TX, USA); SOX9 (Abcam, Massachusetts, USA).

    Techniques: Passaging, Expressing, Immunofluorescence, Staining, Phospho-proteomics, Western Blot